Autofluorescence

Autofluorescence imaging with clinical scanning laser ophthalmoscope systems is routinely performed in the clinic to reveal changes in the deep retinal layers such as retinal pigment epithelium (RPE) and choroid.


In collaboration with Ethan Rossi at University of Pittsburgh, we have modified our adaptive optics scanning laser ophthalmoscope to allow detection of near infrared autofluorescence from retinal RPE cells. Thanks to the dual channel reflectance and fluorescence acquisition, we can detect the photoreceptor and RPE mosaic simultaneously, at a wavelength that is comfortable for subjects.