Full-Field OCT

Full-field optical coherence tomography (FFOCT) is a variation of OCT, in which 2D en face tangential optical slices are directly recorded on a camera without point-by-point lateral scanning. The FFOCT microscope combines the penetration capability and high axial resolution sectioning of OCT with the high transverse resolution of confocal microscopy. Additionally, in FFOCT, contrary to confocal microscopy, axial resolution (dependent on the bandwidth of the light source) and lateral resolution (dependent on the resolving power of the objectives) are completely decoupled. This implies that FFOCT with moderate numerical aperture objectives can achieve a larger field-of-view, along with a long working distance enabling non-contact operation, and at the same time high lateral and axial resolutions down to 1 µm. Recently, another particular feature of FFOCT was discovered, that is, geometrical aberrations do not decrease spatial resolution, but only lower the signal-to-noise ratio (SNR), which can be recovered with adaptive optics. FFOCT has been demonstrated in a variety of applications and is now commercially available. It has been applied to imaging of biopsies of various tissues, studies in developmental biology, in vivo endoscopy, material characterization and, recently, for in vivo human internal fingerprint imaging, useful for personal identification purposes. Regarding ophthalmic applications, FFOCT has been used for the characterization of various ex vivo tissues from the anterior and posterior segments of animal eyes. It was shown to provide valuable assessment of human corneal grafts, which could potentially improve outcome of corneal transplantation.

In-vivo cornea imaging

In-vivo retina imaging

In vivo high-resolution human retinal imaging with wavefront-correctionless full-field OCT.

P Xiao, V Mazlin, K Grieve, JA Sahel, M Fink, AC Boccara.

Optica 5 (4), 409-412.

In vivo high resolution human corneal imaging using full-field optical coherence tomography.

V Mazlin, P Xiao, E Dalimier, K Grieve, K Irsch, JA Sahel, M Fink, C Boccara.

Biomedical Optics Express 9 (2), 557-568.

Stromal striae: a new insight into corneal physiology and mechanics.
Grieve K, Ghoubay D, Georgeon C, Bocheux R, Latour G, Borderie M, Nahas A, Nguyen TM, Schanne-Klein MC, Andreiuolo F, Borderie V.

Scientific Reports 7 (1), 13584 2017.

Cell motility as contrast agent in retinal explant imaging with full-field optical coherence tomography.

Thouvenin O, Boccara C, Fink M, Sahel J, Paques M, Grieve K.

Invest Ophthalmol Vis Sci. 2017;58:4605–4615. DOI:10.1167/iovs.17-22375

En face coherence microscopy.

Thouvenin O, Grieve K, Xiao P, Apelian C, Boccara AC.

Biomed. Opt. Express 2017; 8, 622-639, special issue OCT. Invited paper.

New Parameters in Assessment of Human Donor Corneal Stroma.

Borderie M, Grieve K, Irsch K, Ghoubay D, Georgeon C, De Sousa C, Laroche L, Borderie VM.

Acta Ophthalmol. 2017. doi:10.1111/aos.13351

Full-field OCT technique for high speed event-based optical flow and particle tracking.

Berthelon X, Chenegros G, LibertN, Sahel JA, Grieve K, Benosman R.

Opt Express. 2017. 29;25(11):12611-12621.

Appearance of the retina with full-field optical coherence tomography.
Grieve K, Thouvenin O, Sengupta A, Borderie VM, Paques M.

Invest Ophthalmol Vis Sci. 2016;57:OCT96–OCT104. DOI:10.1167/iovs.15-18856

Imaging Microscopic Features of Keratoconic Corneal Morphology.

Grieve K, Georgeon C, Andreiuolo F, et al.

Cornea. 2016;35(12):1621-1630. doi:10.1097/ICO.0000000000000979.

Three-dimensional structure of the mammalian limbal stem cell niche.

Grieve K, Ghoubay D, Georgeon C, Thouvenin O, Bouheraoua N, Paques M, Borderie VM.

Exp Eye Res. 2015 Nov;140:75-84. doi: 10.1016/j.exer.2015.08.003. Epub 2015 Aug 20.